Journal: Renal Failure
Article Title: Inhibition of caspase-1 by ginsenoside Rg1 ameliorates d -gal-induced renal aging and injury through suppression of oxidative stress and inflammation
doi: 10.1080/0886022X.2025.2504634
Figure Lengend Snippet: Changes of caspase-1 signal pathway in kidney tissue after Rg1 intervention. (A) qRT-PCR analysis of caspase-1 transcription in kidney in each group. (B) qRT-PCR analysis of IL-1 transcription in kidney in each group. (C) qRT-PCR analysis of IL-18 transcription in kidney in each group. (D) Western blots of caspase-1 of kidney tissue. (E) Relative protein expression of caspase-1 in kidney. (F) The level of interleukin-1 (IL-1, pg) in kidney measured by ELISA kit. (G) The level of interleukin-18 (IL-18, ng) in kidney measured by ELISA kit. The data are expressed as the means ± SEM, n = 3. * p < 0.05, ** p < 0.01, ** p < 0.001.
Article Snippet: The enzyme-linked immunosorbent assay (ELISA) Kits utilized in the experiment are as follows: mouse serum creatinine (Scr) ELISA kit (mouse, JONLNBIO, Jiangsu, China, Cat# JL20633, RRID: AB_3661983); mouse Urea Nitrogen (BUN) ELISA Kit (mouse, Sbjbio, Nanjing, China, Cat# JBS-M0549, RRID: AB_3661991); mouse SOD ELISA KIT (mouse, YBIO, Shanghai, China, Cat# IC-SOD1-Mu, RRID: AB_3661984); mouse IL-18 ELISA KIT (mouse, Chuan Qiu Biotechnology, Shanghai, China, Cat# HM-022, RRID: AB_3661985); mouse IL-1 ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1516, RRID: AB_3661986); tumor necrosis factor-α (TNF-α) ELISA KIT (mouse, Bioss, Beijing, China, Cat# bsk12002, RRID: AB_3661987); mouse MDA ELISA KIT (mouse, Westangbio, Shanghai, China, Cat# XY-SJH-XS1331, RRID: AB_3661988); mouse ROS ELISA KIT (mouse, Yan Yu Medical Reagent, Shanghai, China, Cat# YYu-ELISA-374950, RRID: AB_3661989); mouse beta galactosidases (β-GAL) ELISA KIT (mouse, KlBio, Shanghai, China, Cat# kl-M1264c, RRID: AB_3661990).
Techniques: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay